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By Woodruff T.K.

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S. EPA has published a series of test guidelines for the EDSP (59), and issued the test orders for the first set of chemicals to be screened (60). S. , EDSTAC, EDMVS, EDMVAC), the Interagency Coordinating Committee on the Validation of Alternative Methods (11), and SAP reviews. The general public, multiple stakeholders, and the regulated community have participated in open Federal Advisory committee meetings and workshops, as well as in public comment periods advertised in the Federal Register, and have provided valuable input and feedback on the development of the assays for the battery.

The OECD is currently developing a draft PBTG for the ERa transcriptional activation assay (HeLa 9903 cell line) that could ultimately allow ER TA assays to be used with newer technology and/or proprietary components. Finally, information gleaned from emerging technologies in genomics, proteomics, metabolomics, and computational toxicology will likely be incorporated into the second-generation T1S battery. S. gov/ncct/toxcast/), and data generated by the TOX21 initiative (61) will play a significant role in the prioritization and selection of chemicals to be tested in the next phase of the EDSP.

In mammals, the HPT axis is controlled by complex interactions that make it a difficult target for identifying endocrine disruption. To date, environmental chemicals have been shown to alter thyroid receptor signaling and to disrupt thyroid hormone synthesis and homeostasis (52,53). A number of specific sites within the thyroid hormone pathway are susceptible to insult. , thyroperoxidase or iodothyronine deiodinases), or altering iodine uptake, can deplete circulating levels of triiodothyronine (T3) and/or thyroxine (T4).

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